Posted by RAMACHANDRA BHAT on Sep 8, 2018 4:06 am
To understand the excitation wavelength, first record a spectrum (UV-Vis, full range as available). From the spectrum, you could get the absorption maxima, to use as excitation wavelength. You may prefer to test with the lowest wavelength (highest energy) maxima as the first try (if there are many absorption peaks). Because, excitation energy is always higher compared to emission energy. Then, scan the emission spectrum.